C9097-39D
Cytokeratin 7
Size
£785.00
- SKU:
- C9097-39D
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Cytoskeletal proteins from cultured HeLa cells.
- Clone:
- 5F234 (Ks7.18)
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O.
- Extra Details:
- Represents an excellent marker for the discrimination of specific subtypes of adenocarcinoma: e.g. adenocarcinoma of pancreas, bile duct carcinoma and transitional carcinoma of bladder are stained, whereas colorectal, hepatocellular and prostate carcinomas are negative. Detects specific subtypes of adenocarcinomas: Adenocarcimona of pancreas, gallbladder, lung, cervix; cholangio carcinoma of liver; ductal and lobular carcinoma of breast; carcinomas of ovary; transitional cell carcinoma of bladder; mesothelioma; negative with colorectal adeno carcinoma and most cases of hepatocellular carcinoma. Occasionally, staining of blood vessel walls, particularly of endothelial cells may be observed. Applications: Suitable for use in Immunohistochemistry, Western Blot and with Cytological Material. Other applications not tetsed. Recommended Dilutions: Immunohistochemistry (Frozen and Paraffin): 1:50 Dilute with PBS, pH 7.4; Incubation Time 1 hour at RT, extended with paraffin sections (overnight at 4°C). Protease pretreatment is recommended with paraffin sections prior to antibody application. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice