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C9097-29A

Cytokeratin 20 (FITC)

Size

£1,033.00

SKU:
C9097-29A
Purity:
Purified by affinity chromatography.
Storage Conditions:
-20°C
Supplier:
United States Biological
Host:
Mouse
Immunogen:
Electrophoretically purified Cytokeratin (CK) 20 from human intestinal mucosa.
Clone:
5F216
Format:
Supplied as a liquid in PBS, pH 7.4. Labeled with fluorescein isothiocyanate (FITC).
Extra Details:
Represents an excellent marker for certain types of carcinomas such as adenocarcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin. Very sensitive detection of intestestinal and gastric foveolar epithelium, urothelial umbrella cells, Merkel cells of epidermis as well as tumors originating therefrom (e.g. primary and metastatic colorectal carcinoma). Adenocarcinomas of breast, lung, endometrium and ovary (non-mucinous) as well as neuroendocrine tumors of the lung are essentially negative. Applications: Suitable for use in Flow Cytometry, Immunocytochemistry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (Frozen, Paraffin): 1:10-1:20 in PBS, pH 7.4. Incubate for 1 hour at RT or overnight at 2-8ºC. Microwave treatment required for paraffin sections. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Shipping Conditions:
Blue Ice