C9097-24B
Cytokeratin 19
Size
£659.00
- SKU:
- C9097-24B
- Purity:
- Purified
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Cytoskeletal preparation of the human bladder carcinoma cell line T24.
- Clone:
- 4A36 (RCK108)
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. No stabilizing proteins added.
- Extra Details:
- Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40kD and 68kD and isoelectric pH between 4.9-7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry and Flow Cytometry. Other applications not tested. Recommended Dilutions: Western Blot: 1:100-1:1000 Immunohistochemistry (Frozen, paraffin): 1:100-1:200 using avidin-biotinylated HRP complex (ABC) for detection. Paraffin tissues require pretreatment with pepsin or trypsin. Flow Cytometry: 1:100-1:200 Optimum dilutions to be determined by researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice