C9097-23D1
Cytokeratin 18
Size
£902.00
- SKU:
- C9097-23D1
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human cytokeratin 18 from HeLa cytoskeletal preparation.
- Clone:
- 5F227 (Ks18.04)
- Format:
- Supplied as a lyophilized powder in PBS, pH 7.4, 0.5% BSA and 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O.
- Extra Details:
- Represents an excellent marker to discriminate simple epithelia from those of different origin. Tumors specifically detected: all adenocarcinoma; mammary carcinoma, urinary bladder carcinoma, undifferentiated carcinoma, cervix carcinoma, hepatocellular carcinoma. Polypeptide reacting: Mr 45 000 polypeptide (human cytokeratin 18) of all simple type epithelia and basal cells of many squamous, nonepidermal epithelia. Applications: Suitable foruse in Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilution: Immunohistochemistry (frozen and paraffin-embedded tissue and cytological material): 1:20; Incubation Time 1h at 37°C, extended with paraffin sections microwave treatment is recommended (overnight at 4°C). Positive Controls: HeLa and MCF-7. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice