C9097-13F1

Cytokeratin 14 (Cytokeratin-14, CK14, CK-14, EBS3, EBS4, Keratin 14, Keratin-14, Keratin Type I Cytoskeletal 14, K14, KRT14, NFJ) (FITC)

Size

£633.00

SKU:: C9097-13F1
Purity:: Purified by Protein G affinity chromatography from tissue culture supernatant.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Last 15 C-terminal residues of human cytokeratin 14 conjugated to thyroglobulin.
Clone:: 3H2053 (LL002)
Format:: Supplied as a liquid in PBS, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Extra Details:: Cytokeratin 14 is a type I intermediate filament, expressed by stratifying epithelial cells and can be used to distinguish these cell types from simple epithelial cells, which do not express cytokeratin 14. Applications: Suitable for use in Flow Cytometry and Immunofluorescence. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat, 1:10, 10ul labels 10e6 cells in 100ul. Membrane permeabilization is required for this application Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. Do not freeze. For long-term storage, aliquot to avoid repeated freezing and thawing and store at 4°C. Aliquots are stable at 4°C for 12 months after receipt. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.