C9097-12C

Cytokeratin 13

Size

£800.00

SKU:: C9097-12C
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Cytokeratin 13 of Mr 54,000 purified from human esophagus.
Clone:: 5F225
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O.
Extra Details:: Represents an excellent marker to discriminate non-cornified squamous epithelia from those of different origin. Applications: Suitable for use in Immunofluorescence, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilution: Immunohistochemistry (Frozen, paraffin-embedded tissue and cytological): 1:10, Protease pretreatment improves the performance with PBS, pH 7.4; Incubation Time 1 hr at RT; extended with paraffin. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.