C9097-11D
Cytokeratin 10
Size
£659.00
- SKU:
- C9097-11D
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Cytoskeletal preparation extracted from human epidermis.
- Clone:
- 4A27 (DE-K10)
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. No stabilizing proteins added.
- Extra Details:
- Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40kD and 68kD and isoelectric pH between 4.9-7.8. The individual cytokeratin polypeptides are designated 1 to 20. Cytokeratin 1 has the highest molecular weight and the highest isoelectric pH, while cytokeratin 19 has the lowest molecular weight and a low isoelectric pH. The cytokeratins are divided into the type I and type II subgroups, the type II family members comprising the basic to neutral cytokeratins 1-8, while the type I group comprises the acidic cytokeratins 9-20. The cytokeratins are expressed in combinations which characterize the type of epithelium. Applications: Suitable for use in Immunohistochemistry, Flow Cytometry, Immunocytochemistry and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50-1:100 Immunohistochemistry (frozen and paraffin sections): 1:50-1:100 using ABC detection reagent. Western Blot: 1:50-1:500 Optimum dilutions to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice