C9097-04

Cytokeratin 1,10

Size

£633.00

SKU:: C9097-04
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Keratin 1 and 10.
Clone:: 2Q1048 (LH1)
Format:: Supplied as a liquid in PBS, 0.1% BSA, 0.09% sodium azide.
Extra Details:: Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40kD and 68kD and isoelectric pH between 4.9-7.8. The individual cytokeratin polypeptides are designated 1 to 20. Cytokeratin 1 has the highest molecular weight and the highest isoelectric pH, while cytokeratin 19 has the lowest molecular weight and a low isoelectric pH. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: Formalin fixed sections. Note: Pretreatment with type XXIV protease (0.5mg/ml) produces best results. HIER (Microwave citrate buffer pH 6.0) produces some staining. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.