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C9095-17W

Cytochrome P450 2E1

Size

£570.00

SKU:
C9095-17W
Application:
WB
Purity:
Purified
Storage Conditions:
-20°C
Supplier:
United States Biological
Host:
Rabbit
Immunogen:
Recombinant protein corresponding to human P450 2E1 expressed in E. coli
Format:
Supplied as a liquid in 20mM phosphate buffer, pH 8.0.
Extra Details:
Cytochrome P450 is mainly found in liver, and can metabolize a wide range of compounds such as organic solvents, acetaminophen, dimethylnitrosoamine and aliphatic alcohol, which have certain toxicological effects in humans. Ethanol-induced cytochrome P450 IIE1 is considered to be one of the major causes of oxidative stress in the liver following ethanol treatment. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 will yield a positive result with ≥0.2ug of recombinant human cytochrome P450 2E1 using alkaline phosphatase-conjugated anti-rabbit IgG secondary antibody. Optimum dilutions to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Shipping Conditions:
Blue Ice