C9095-15T

Cytochrome P450 2B1,2B2

Size

£557.00

SKU:: C9095-15T
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Clone:: 4i184
Format:: Supplied in liquid.
Extra Details:: Phase 1 drug metabolism is catalyzed by two groups of monooxygenases, the cytochrome P450s (CYPs) and the flavin monooxygenases (FMOs). Both CYPs and FMOs are localized in the endoplasmic reticulum. Collectively, these enzymes catalyze the oxidation of a very broad range of substrates. Both classes are dependent on NADPH as a source of electrons, but the two classes exhibit significant differences in mechanism and substrate specificity. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:100 yields a strong band with 10ug of phenobarbital induced rat microsomes using alkaline phosphatase. Optimum dilutions to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.