C9095-07A
Cytochrome C Releasing Factor 21 (CRF21)
Size
£843.00
- SKU:
- C9095-07A
- Application:
- WB
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Sheep
- Immunogen:
- Recombinant human Cytochrome c-releasing factor 21, Met1-Leu188, expressed in E.coli.
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- CRF21 (Cytochrome c-releasing factor 21; also C7orf24 or LOC79017) is a 21kD AIG2-like family member. It is a cytosolic protein that induces the release of Cytochrome c from mitochondria, possibly initiating apoptosis. Human CRF21 is 188aa in length and is a complex of beta-sheets and alpha-helices that create two terminal domains. It has been proposed that CRF21 forms homodimers. There are at least two possible splice variants of human CRF21, one showing a 70aa substitution, while the second variant shows an 18aa substitution for aa97-188. Full-length human CRF21 is 82% aa identical to mouse CRF21. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1.0ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice