C9095-04A-FITC
Cytochrome C (FITC)
Size
£1,042.00
- SKU:
- C9095-04A-FITC
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Rat cytochrome C.
- Clone:
- 3G119
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Fluorescein isothiocyanate (FITC).
- Extra Details:
- Cytochrome c is a requisite component of the mitochondrial respiratory chain and participates in the electron transfer between complex III and complex IV of the respiratory chain. Cytochrome c is a soluble protein that is localized in the mitochondrial intermembrane space and is loosely attached to the surface of the inner membrane. The protein is synthesized on cytoplasmic ribosomes as apocytochrome c and then translocated into the mitochondria via a unique pathway independent of the general protein translocation machinery, protease-sensitive components of the outer membrane, or a membrane potential across the inner membrane. In response to a variety of apoptotic stimuli, Cytochrome c is released from mitochondria into the cytosol. This release can be blocked by Bcl-2 and suggests that Bcl-2 may function by regulating Cytochrome c release. Cytosolic Cytochrome c is an essential component of the vertebrate apoptosome which is composed of Cytochrome c, Apaf-1, and procaspase-9 (Apaf-3). Interaction of these proteins leads to the activation of caspase-9, which in turn activates other caspases (such as caspase-3) ultimately resulting in cell death. The current working hypothesis is that release of Cytochrome c from mitochondria commits a cell to die either by a “rapid” apoptotic mechanism involving activation of the Apaf-1/caspase-9 cascade, or by a “slower” necrotic process involving the collapse of the electron transport chain. Applications: Suitable for use in Immunofluorescence, FLISA and Immunoprecipitation. Other applications not tested. Recommended Dilution: FLISA (Native): 0.1-1ug/ml Immunofluorescence: 20ug/ml Immunoprecipitation (Native): 15ug (for extract from ~10e6 cells) Optimal dilutions to be determined by the researcher. Positive Control: HeLa, Rat6 Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice