C9095-02K

Cytochrome C (CYCS, CYC)

Size

£301.00

SKU:: C9095-02K
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Rat cyt c-OVA
Clone:: 6H2.B4
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Extra Details:: Cytochrome c is a 15kD protein found in the mitochondrial intermembrane space with a heme-binding domain. Cytochrome c is a component of the electron transport chain; the heme group transfers electrons from cytochrome b-c1 complex to cytochrome oxidase complex. Cytochrome c initiates apoptosis by release to cytoplasm and binding Apaf-1 which activates procaspase 9. Cytochrome c interacts with the cytochrome b-c1 complex, cytochrome oxidase complex, heme, Apaf-1, and Caspase 9 proteins. Applications: Suitable for use in Flow Cytometry, Immunocytochemistry, Immunofluorescence and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Flow Cytometry: ≤0.5ug/10e6 cells in 100ul volume Western Blot: 1:1000 Immunoprecipitation: 2-4ug/1x10e7 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.