C9095-02C

Cytochrome C (CYC, Cytochrome C Somatic, CYCS, HCS, THC4) (Biotin)

Size

£505.00

SKU:: C9095-02C
Application:: WB
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Synthetic peptides corresponding to aa1-80, 81-104 and 66-104 of pigeon CYT. Epitope: Within aa93-104 of pigeon CYT.
Clone:: 7H8.2C12
Format:: Supplied as a liquid in PBS. No preservative and no stabilizing protein added. Labeled with Biotin.
Extra Details:: In addition to its well-known role in electron transport, cytochrome c (CYT) has been identified as a mediator of apoptosis or programmed cell death. Cytochrome c translocates from mitochondria into the cytoplasm in the early phases of apoptosis and plays a role in initiation of the apoptotic proteolytic cascade by activating caspase-3. Cytochrome c has been shown to bind Apaf-1. Apaf-1 has also been shown to activate capase-3. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:250-1:500 Optimal dilutions to be determined by the researcher. Positive Control: HeLa cells lysate Storage and Stability: Store at 4°C. Do Not Freeze. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.