C9094-34B

CYBB (Cytochrome b-245 Heavy Chain, CGD91-phox, Cytochrome b(558) Subunit beta, Cytochrome b558 Subunit beta, Heme-binding Membrane Glycoprotein gp91phox, NADPH Oxidase 2, Neutrophil Cytochrome b 91kD Polypeptide, Superoxide-generating NADPH Oxidase Heavy Chain Subunit, gp91-1, gp91-phox, p22 Phagocyte B-cytochrome, NOX2)

Size

£642.00

SKU:: C9094-34B
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Synthetic peptide corresponding to C-SYLNFARKRIKNP, from human CYBB, at the internal region of the protein (NP_000388.2). Species sequence homology: canine
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Extra Details:: The integral membrane protein flavocytochrome b (Cyt b), a heterodimer formed by a 91kD glycoprotein (heavy chain) and a 22kD polypeptide (light chain), is the catalytic core of the phagocyte NADPH oxidase and generates superoxide which plays a critical role in host defense. NADPH oxidase is a central well characterized component of the innate immune response against fungal and bacterial infections. Recent research also offers new insight into a novel role for this oxidase in the coordination of the adaptive immune response. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:2000 Western Blot: 0.03-0.1ug/ml, observed in human peripheral blood mononucleocytes lysates on ~85kD bands Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice