C8855-30
Cyclophilin B, Recombinant (PPIB, Peptidylprolyl isomerase B (Cyclophilin B), CYPB, CYP-S1, Peptidyl-prolyl cis-trans isomerase B, PPIase, Rotamase, S-cyclophilin, SCYLP)
Size
£492.00
- SKU:
- C8855-30
- Molecular Weight:
- 21.2
- Purity:
- ≥95% (SDS-PAGE). Endotoxin: ≤1EU/ug (LAL).
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Format:
- Supplied as a liquid in 20mM Tris, pH 8.0, 20mM sodium chloride, 0.5mM DTT, 10% glycerol.
- Extra Details:
- Cyclophilin B (also known as PPIB, peptidylpropyl isomerase B) is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine Amediated immunosuppression. Recombinant human cyclophilin B was expressed in E. coli and purified by using conventional chromatography techniques Recombinant protein corresponding to aa26-216 from human cyclophilin B, expressed in E. coli. Accession: NP_000933 Molecular Weight: ~21.2kD (192aa) confirmed by MALDI-TOF Amino Acid Sequence: MLLPGPSAAD EKKKGPKVTV KVYFDLRIGD EDVGRVIFGL FGKTVPKTVD NFVALATGEK GFGYKNSKFH RVIKDFMIQG GDFTRGDGTG GKSIYGERFP DENFKLKHYG PGWVSMANAG KDTNGSQFFI TTVKTAWLDG KHVVFGKVLE GMEVVRKVES TKTDSRDKPL KDVIIADCGK IEVEKPFAIA KE Enzyme Activity: Specific activity is > 1,500 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAFP-PNA per minute at 37°C in Tris-HCl pH 8.0 using chymotrypsin. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice