C8855-01B

Cyclophilin B (PPIB, Peptidylpropyl Isomerase B) [KO Validated]

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£869.00

SKU:: C8855-01B
Purity:: Purified by Protein A affinity chromatography from hybridoma culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to Asp34-Glu216 from human Cyclophilin B, expressed in E. coli.
Clone:: 9H108
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: Cyclophilin B is a 24kD glycoprotein member of the B subfamily of the cyclophilin-type PPIase family of molecules. It is both secreted and retained in the ER. When secreted, Cyclophilin B mediates chemotaxis and T cell adhesion to fibronectin. This is likely due to its prolyl cis/trans isomerase activity. Intracellularly, Cyclophilin B appears to serve as a molecular chaperone for molecules destined for secretion. It does so via stabilization, and facilitating the activity of additional chaperones. The human Cyclophilin B precursor is 216 amino acids (aa) in length. It contains a 25aa signal sequence plus a 191aa mature region. There is a partial heparin-binding sequence (aa27-34), a PPIase domain (aa47-204) and a C-terminal ER retention motif (aa213-216). Applications: Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml detects Cyclophilin B in HeLa human cervical epithelial carcinoma cell line, A431 human epithelial carcinoma cell line, BaF3 mouse pro-B cell line, PC-12 rat adrenal pheochromocytoma cell line, Rat-2 rat embryonic fibroblast cell line and NRK rat normal kidney cells. Immunocytochemistry: 8-25ug/ml. Immersion fixed HeLa human cervical epithelial carcinoma cell line Knockout Validated: Cyclophilin B is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in Cyclophilin B knockout HeLa cell line. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice