C8455-05

Cyclin E, p52

Size

£793.00

SKU:: C8455-05
Purity:: Purified by Protein G affinity chromatography from ascites.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human Cyclin E.
Clone:: 0.T.206 (HE12)
Format:: Supplied as liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide
Extra Details:: Cyclin E is a regulatory subunit of cdk-2 and controls G1/S transition during the mammalian cell cycle. Multiple isoforms of cyclin E are expressed only in tumors but not in the normal tissues. Qualitative and quantitative alterations in cyclin E protein have been implicated as indicators of worse prognosis in various cancers. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.5-2ug/ml detects cyclin E (~52kD) in 20ug of human A431 lysate. Non-stimulated A431 cell lysate was resolved by electrophoresis, transferred to PVDF and probed with C8455-05 (1ug/ml). Proteins were visualized by using an IgG (HRP) goat anti-mouse secondary antibody and a chemiluminescence detection system. Immunoprecipitation: 4ug immunoprecipitated cyclin E from 500ug of human A431 RIPA cell lysate as confirmed by Western Blot analysis of the immunoprecipitate using rabbit polyclonal anti-cyclin E. Immunocytochemistry: 10ug/ml detected cyclin E in 50% ethanol/50% acetic acid-fixed human A431 cells. Optimal dilutions to be determined by the researcher. Positive Antigen Control (Included): A0001-50: Non-stimulated A431 cell lysate Recommended Secondary Antibodies: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo 1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab Gt xMo I1904-23: IgG, X-Adsorbed (DTAF) Pab Gt xMo Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice