C8455-05-ML650
Cyclin E, p52 (MaxLight 650)
Size
£1,023.00
- SKU:
- C8455-05-ML650
- Purity:
- Purified by Protein G affinity chromatography from ascites.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to human Cyclin E.
- Clone:
- 0.T.206 (HE12)
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
- Extra Details:
- MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. Cyclin E is a regulatory subunit of cdk-2 and controls G1/S transition during the mammalian cell cycle. Multiple isoforms of cyclin E are expressed only in tumors but not in the normal tissues. Qualitative and quantitative alterations in cyclin E protein have been implicated as indicators of worse prognosis in various cancers. Applications: Suitable for use in FLISA, Western Blot, Immunoprecipitation, and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: detects cyclin E (~52kD) in 20ug of human A431 lysate. Non-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with C8455-05 (0.5ug/ml). Proteins were visualized by using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 4ug immunoprecipitated cyclin E from 500ug of human A431 RIPA cell lysate as confirmed by Western blot analysis of the immunoprecipitate using rabbit polyclonal anti-cyclin E. Immunocytochemistry: detected cyclin E in 50% ethanol/50% acetic acid-fixed human A431 cells. Optimal dilutions to be determined by the researcher. Positive Antigen Control (Included): A0001-50 (Non-stimulated A431 cell lysate). I*Recommended secondary antibodies: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo 1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab Gt xMo I1904-23: IgG, X-Adsorbed (DTAF) Pab Gt xMo Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice