C8455-05-BIOTIN
Cyclin E, p52 (Biotin)
Size
£1,023.00
- SKU:
- C8455-05-BIOTIN
- Purity:
- Purified by Protein G affinity chromatography from ascites.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to human Cyclin E.
- Clone:
- 0.T.206 (HE12)
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
- Extra Details:
- Cyclin E is a regulatory subunit of cdk-2 and controls G1/S transition during the mammalian cell cycle. Multiple isoforms of cyclin E are expressed only in tumors but not in the normal tissues. Qualitative and quantitative alterations in cyclin E protein have been implicated as indicators of worse prognosis in various cancers. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: detects cyclin E (~52kD) in 20ug of human A431 lysate. Non-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with C8455-05 (0.5ug/ml). Proteins were visualized by using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 4ug immunoprecipitated cyclin E from 500ug of human A431 RIPA cell lysate as confirmed by Western blot analysis of the immunoprecipitate using rabbit polyclonal anti-cyclin E. Immunocytochemistry: detected cyclin E in 50% ethanol/50% acetic acid-fixed human A431 cells. Optimal dilutions to be determined by the researcher. Positive Antigen Control (Included): A0001-50 (Non-stimulated A431 cell lysate). I*Recommended secondary antibodies: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo 1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab Gt xMo I1904-23: IgG, X-Adsorbed (DTAF) Pab Gt xMo Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice