C8454-20X

Cyclin D3) (CCNE 1, CCNE, Cyclin E1, Cyclin Es, Cyclin Et, G1/S specific cyclin E, CCND3) (BSA & Azide Free

Size

£960.00

SKU:: C8454-20X
Purity:: Purified by Protein A/G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified human recombinant full length cyclin D3 protein.
Clone:: DCS-22
Format:: Supplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See C8454-20.
Extra Details:: Applications: Suitable for use in Flow Cytometry, Immunofluorescence, Immunoprecipitation (will not IP denatured antigen), Western Blotting, Immunohistochemistry (frozen and methanol/Carnoy’s or formalin/paraffin). Not recommended for Kinase Assay or Neutralization. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min., followed by cooling at RT for 20 min. Recommended Dilution: Immunoprecipitation: Use Protein G; 2ug antibody/mg protein lysate Western Blotting: 1ug/ml Immunohistochemistry: 2-4ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.