C8454-20A

Cyclin D3 (CCNE 1, CCNE, Cyclin E1, Cyclin Es, Cyclin Et, G1/S specific cyclin E, CCND3)

Size

£607.00

SKU:: C8454-20A
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to cyclin D3 of human origin and maps to residues 241-260.
Clone:: 3i12(DCS22)
Format:: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol, less than 0.02% sodium azide.
Extra Details:: Activity of the cyclin-dependent kinases cdk4 and cdk6 is regulated by T-loop phosphorylation, by the abundance of their cyclin partners (the D-type cyclins), and by association with cdk inhibitors of the Cip/Kip or INK family of proteins (1). In growing cells, the inactive ternary complex of cyclin D/cdk4 and p27 Kip1 requires extracellular mitogenic stimuli for the release and degradation of p27 concomitant with a rise in cyclin D levels to effect progression through the restriction point in a pRb-dependent entry into S-phase (2). The active complex of cyclin D/cdk4 targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1Sphase gene expression (3). Levels of cyclin D protein drop upon withdrawal of growth factors through downregulation of its protein expression and through phosphorylation-dependent degradation (4). Although the Dtype cyclins are not fully redundant, cyclin D3, like D1, plays a prominent role in differentiation as well as a proliferative role, which correlates with higher expression levels of cyclin D3 in various cancers (5). Applications: Suitable for use in Western Blot, Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:2000 Immunohistochemistry (Paraffin): 1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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