C8454-06A-FITC
Cyclin D1 (FITC)
Size
£1,042.00
- SKU:
- C8454-06A-FITC
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Full length human cyclin D1 protein.
- Clone:
- 3G112
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Fluorescein isothiocyanate (FITC).
- Extra Details:
- Cyclin D1 is a 36kD nuclear protein. The cell cycle in eukaryotic cells is mediated by the formation, activation, and deactivation of complexes containing Cyclin-Dependent Kinases (CDK) and cyclins. D-type cyclins usually associate with CDK4 (p34 PSK-J3). Complexes with CDK2 and CDK5 have also been reported (1). D-type cyclins are necessary for cells to achieve the G1 to S transition in the cell cycle (2). The D-type cyclins have been shown to associate with PCNA, the retinoblastoma gene product, p21, and other proteins. Applications: Suitable for use in FLISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested. Recommended Dilution: FLISA: 0.1-1.0ug/ml Western Blot: 0.3-1.0ug/ml Immunoprecipitation: 5ug Immunohistochemistry: 2-10ug/ml. Formalin-fixed paraffin embedded sections require a Heat Induced epitope Retrieval (HIER) step prior to staining. Optimal dilutions to be determined by the researcher. Source: Ascites Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice