C8454-02D

Cyclin D1 (PRAD-1, bcl 1)

Size

£812.00

SKU:: C8454-02D
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: E. coli-derived recombinant human Cyclin D1 (aa1-295) Accession # NP_444284
Clone:: 7H91
Format:: Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
Extra Details:: Cyclin D1 is a 44kD cell cycle regulatory protein whose expression level and nuclear/cytoplasmic distribution are tightly regulated in synchrony with the cell cycle. Cyclin D1 complexes containing Cdk4 or Cdk6 induce phosphorylation of Rb, a requirement for progression through the G1/S cell cycle transition. Human Cyclin D1 shares 93% amino acid sequence identity with mouse and rat Cyclin D1. Applications: Suitable for use in Immunohistochemistry and ELISA. Other applications not tested. Recommended Dilution: Immunohistochemistry: 10ug/ml ELISA: 0.5-1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.