C8454-01D
Cyclin D1
Size
£735.00
- SKU:
- C8454-01D
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human recombinant full-length cyclin D1 protein.
- Clone:
- 5F184 (DCS-6)
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O.
- Extra Details:
- Cyclin D1 gene is identical to the BCL-1 and the PRAD-1 oncogene and is part of the chromosome 11q13 amplicon which is amplified in several common tumor types like breast, lung, esophagus, urinary bladder and head and neck carcinoma. This antibody is especially useful in tumor diagnosis: it can be used as fast method to identify cases with 11q13 amplifications. Polypeptide reacting: Mr 36,000 polypeptide (cyclin D1 polypeptide of human cells). Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Flow Cytometry and Neutralization. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Frozen and paraffin): 1:10 with PBS, pH 7.4; Incubation Time 1 hr at RT or overnight at 4°C for paraffin sections. Paraffin without microwave treatment only after fixation in methacarn, ethanol or phenol formalin Western Blot: 1:25-1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice