C8452-71B

Cyclin A1 (Cyclin-A1, CCNA1)

Size

£832.00

SKU:: C8452-71B
Purity:: Purified by Protein G affinity chromatography from hybridoma culture supernatant
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to Lys51-Arg220 from human Cyclin A1, expressed in E. coli.
Clone:: 12K76
Format:: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: Cyclin A1, also known as CCNA1, is a 64kD protein that associates with the kinase Cdk2. This complex phosphorylates multiple substrates involved in regulation of cell cycle progression, apoptosis, double strand DNA break repair, and mRNA splicing. Cyclin A1 activity promotes G2/M phase transition in spermatocyte meiosis and G1/S transition in somatic cells. Cyclin A1 is upregulated in myeloid leukemia, testicular germ cell tumors, and several carcinomas. It contains two cyclin box folds aa243-318 and 340-427. Over aa51-220, human Cyclin A1 shares approximately 75% aa identity with mouse and rat Cyclin A1. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml using THP-1 human acute monocytic leukemia cell line, HeLa human cervical epithelial carcinoma cell line, NTera-2 human testicular embryonic carcinoma cell line, MCF-7 human breast cancer cell line, HEK293 human embryonic kidney cell line, and human testis tissue Immunohistochemistry: 8-25ug/ml using immersion fixed paraffin-embedded sections of human testis Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice