C8450-09A

Cyclic AMP Dependent Protein Kinase Regulatory Subunit 1b (cAMP PKA R1b)

Size

£882.00

SKU:: C8450-09A
Application:: WB
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Sheep
Immunogen:: Recombinant protein corresponding to Met1-Val381 of human PKA RI beta, expressed in E. coli.
Format:: Supplied as a lyophilized powder in PBS with 5% trehalose. Reconstitute with sterile PBS.
Extra Details:: The cAMP-dependent protein kinase (PKA) holoenzyme is composed of two regulatory and two catalytic subunits, designated PKA R and PKA C, respectively. PKA R subunits exist as two classes, RI and RII, with each class containing two isoforms, α and β. Upon PKA R subunit binding to the second messenger cAMP, active PKA C subunits are released, initiating a phosphorylation cascade that regulates such cellular functions as metabolism, ion transport, and gene transcription. Applications: Western Blot: 1.0ug/ml Optimal dilutions should be determined by each laboratory for each application. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile PBS and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.