C8375-12D

CXCR5 (C-X-C Chemokine Receptor 5)

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£837.00

SKU:: C8375-12D
Purity:: Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Recombinant protein corresponding to Met1-Pro57 from mouse CXCR5 expressed in E. coli.
Clone:: 11D21
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: CXCR5 is a 55-60kD member of the G-protein coupled receptor 1 family. It is expressed on select cell types, including granule and Purkinje cell neurons, embryonic CD4+ CD3- IL-7Ralpha+ precursor cells, B cells and follicular T helper cells. CXCR5 selectively binds BLC/CXCL13. This appears to both promote embryonic lymph node development and, in the adult, direct expressing cells to positions that optimize antigen presentation and antibody production. Mouse CXCR5 is a 7-transmembrane glycoprotein that is 374 amino acids (aa) in length. It contains a 57aa N-terminal extracellular region plus a 47aa C-terminal cytoplasmic domain. Over aa1-57, mouse CXCR5 shares 84% and 47% aa identity with rat and human CXCR5, respectively. Applications: Suitable for use in Flow Cytometry, Direct ELISA and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 0.25ug/10e6 cells. Detects CXCR5 in mouse splenocytes and T follicular helper cells Immunocytochemistry: 8-25ug/ml. CXCR5 was detected in immersion fixed mouse splenocytes and immersion fixed A20 mouse B cell lymphoma cells. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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