CXCR5 (C-X-C Chemokine Receptor 5, CD185)

Size

£889.00

SKU:: C8375-12C
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Recombinant protein corresponding to Met1-Pro57, Glu112-Thr126, Lys191-Phe221, and Leu283-Ile306 from mouse CXCR5 joined by linker peptides and expressed in E. coli.
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: CXCR5 (CXC chemokine receptor 5; also BLR-1, NLR and CD185) is a 55-60kD member of the G-protein coupled receptor 1 family. It is expressed on select cell types, including granule and Purkinje cell neurons, embryonic CD4+ CD3- IL-7Ra precursor cells, B cells and follicular T helper cells. CXCR5 selectively binds BLC/CXCL13. This appears to both promote embryonic lymph node development and, in the adult, direct expressing cells to positions that optimize antigen presentation and antibody production. Mouse CXCR5 is a 7-transmembrane glycoprotein thatis 374(aa) in length. It contains a 57 aa N-terminal extracellular region plus a 47aa C-terminal cytoplasmic domain. Over aa1-57, mouse CXCR5 shares 84% and 47% aa identity with rat and human CXCR5, respectively. Applications: Suitable for use in Flow Cytometry and Direct ELISA. Other applications not tested. Recommended Dilutions: Flow Cytometry: 2.5ug/10e6 cells. Detected CXCR5 in mouse splenocytes. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice