C8375-01E
CXCR4 (Fusin, LESTR, HUMSTR, C-X-C Chemokine Receptor 4, CD184)
Size
£876.00
- SKU:
- C8375-01E
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- 3T3 cells transfected with human CXCR4
- Clone:
- 8J1097
- Format:
- Supplied as a lyophilized powder in PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- CXCR4, also termed Fusin, LESTR, or HUMSTR is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains and functions as a coreceptor for X4 HIV-1 entry into CD4+ cells. CXCR4 is expressed predominantly on naïve T cell subset of peripheral blood and is rapidly upregulated by PHA and IL-2 stimulation. It is also expressed on neutrophils, monocytes and various cell lines. Applications: Suitable for use in Blocking/Neutralization, Flow Cytometry and Immunohistochemistry. . Other applications not tested. Recommended Dilution: Neutralization: Measured by its ability to neutralize CXCL12/SDF‑1 alpha -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR4. The Neutralization Dose (ND50) is typically 2.5-12ug/mL in the presence of 1 ng/mL Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF‑1 alpha. Flow Cytometry: 0.25ug/10e6 cells Immunohistochemistry (Paraffin): 8-25ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice