C8353X-ML550
CXCR4 (Fusin, LESTR, HUMSTR, C-X-C Chemokine Receptor 4, CD184) (BSA & Azide Free) (MaxLight 550)
Size
£1,164.00
- SKU:
- C8353X-ML550
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide derived from the second extracellular loop of human CXCR4. Epitope: Extracellular. MW of Antigen: 40kD.
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
- Extra Details:
- MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000. CXCR4, also termed Fusin, LESTR, or HUMSTR is a member of the G-protein-coupled chemokine receptor family with seven membranespanning domains and functions as a coreceptor for X4 HIV-1 entry into CD4+ cells. CXCR4 is expressed predominantly on naïve T cell subset of peripheral blood and is rapidly upregulated by PHA and IL-2 stimulation. It is also expressed on neutrophils, monocytes and various cell lines. Applications: Suitable for use in Flow Cytometry and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot:1ug/ml for 2 hrs at RT. Optimal dilutions to be determined by the researcher. Positive Control: U937 or HeLa cells Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20°C. Aliquots are stable for at least 12 months at-20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice