C8297-98H1
CXCL5 (Chemokine (C-X-C Motif) Ligand 5, Epithelial Derived Neutrophil Activating Peptide 78, Epithelial Derived Neutrophil Activating Protein 78, ENA78, ENA-78, Neutrophil Activating Peptide ENA-78, Small Inducible Cytokine Subfamily B Member 5, Small Inducible Cytokine B5, SCYB5) (Biotin)
Size
£604.00
- SKU:
- C8297-98H1
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to aa5-78 from human CXCL5, expressed in E. coli.
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.2. No preservative added. Labeled with Biotin. Reconstitute with 500ul sterile ddH2O, 0.1% BSA, PBS.
- Extra Details:
- CXCL5 signals through binding to CXC receptor 2 (CXCR2) and occurs naturally in three isoforms: ENA 5-78 (74aa), ENA 9-78 (70aa) and ENA 10-78 (69aa). Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA (sandwich): using 100ul/well antibody solution use a concentration of 0.25-1ug/ml. In conjunction with PC8297-98H as a capture antibody, allows the detection of at least 0.2-0.4ng/well of recombinant hENA-78. Western Blot: 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hENA-78 is 1.5-3ng/lane, under either reducing or non-reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice