CTHRC1 (Collagen Triple Helix Repeat Containing 1, Collagen Triple Helix Repeat-containing Protein 1) (Biotin)

Size

£908.00

SKU:: C7979-02
Application:: WB
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Sheep
Immunogen:: Recombinant protein corresponding to Ser33-Lys245 of mouse CTHRC1, expressed in NS0 cells. Species Sequence Homology: rat; 99%, human; 93%
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, BSA. Labeled with Biotin. Reconstitute with 250ul sterile PBS.
Extra Details:: CTHRC1 (Collagen triple helix repeat-containing protein 1) is a 28-30kD, secreted glycoprotein that bears similarity to the C1q/TNFa-related family of proteins. It is expressed by disparate cell types, including renal epithelium, neurons, osteoblasts and smooth muscle cells. Functionally, it is recognized to be induced by BMP-2, and to block TGFbeta-induced collagen type I and III synthesis. Mouse CTHRC1 is 245aa in length. It contains a 32aa signal sequence, a 16aa prosegment (aa33-48), and a 197aa mature region that shows one collagen-like domain (aa59-92). Proteolytic processing may generate multiple CTHRC1 isoforms. There is the potential for an intracellular full-length 33kD, 245aa form, plus extracellular isoforms that are 28kD (aa33-245), 26kD (aa49-245), 20kD (aa98-245) and 18kD and 16kD in size, the last two representing variants of the 20kD form with C-terminal processing. CTHRC1 may undergo dimerization, trimerization and oligomerization. One mouse splice variant shows a deletion of aa53-126. Full-length mouse CTHRC1 shares 99% and 93% aa identity with rat and human CTHRC1, respectively. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 0.1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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