C7949-13-ML650
Cullin 2 (CUL-2) (MaxLight 650)
Size
£1,106.00
- SKU:
- C7949-13-ML650
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide derived from the C-terminus of the human CUL2.
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™650.
- Extra Details:
- MaxLight™ 650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. In yeast, proteolysis of G1 cell cycle regulatory proteins is controlled by a ubiquitin ligase formed by three subunits: Cdc53 (also known as CulA), Skp1 and one of many F-box proteins (reviewed in ref 2). A family of human cullin genes homologous to the S. cerevisiae cdc53 gene has been identified (cul1, 2, 3, 4a, 4b, and 5) (1) . It was then shown that Cul1 forms a complex with human Skp1 and the F box protein Skp2. In contrast, Cul2 does not associate with Skp1 and Skp2. Instead it forms a complex with an inactive transcriptional elongation complex, SIII, formed by three subunits: Elongin C, Elongin B and VHL (2) . The meaning of this association is still unknown. Intriguingly, Elongin C shows homology with Skp1 and Elongin B with ubiquitin. In addition, in its active form, SIII contains, instead of VHL, an F box protein: Elongin A. Applications: Suitable for use in FLISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: FLISA (Immunogen): 0.1-1.0ug/ml Western Blot: 1-3ug/ml Immunoprecipitation (Native): 5-10ug Optimal dilutions to be determined by the researcher. Positive Control: CUL2 recombinant protein and cell lysates Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice