C7948-50C
CUG-Binding Protein 1 (CUG-BPI, Cytidine Uridine Guanosine Binding Protein 1)
Size
£927.00
- SKU:
- C7948-50C
- Purity:
- Purified immunoglobulin
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Purified rat CUG-BP1 protein
- Clone:
- 7C23
- Format:
- Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
- Extra Details:
- RNA editing is an important mechanism for regulating genetic plasticity through the generation of alternative protein productsfrom a single structural gene. Substitutional RNA editing employsa variety of genetic mechanisms, the biochemical basis of whichhas been elucidated following the development of in vitro assaysthat recapitulate important elements of this process. There are two typesof substitutional RNA exist in mammals, namely A-to-I and C-to-URNA editing. The best-characterized example of C-to-U RNA editing involves the nuclear transcript encoding intestinal apolipoprotein B(apo B)). Apo B RNA editing changes a CAA to a UAA stop codon,generating a truncated protein, apoB48. The functional complex includes a minimal corecomposed of apobec-1 and ACF, that functionas an adaptor protein by binding both the deaminase and the RNAsubstrate. The RNA binding proteins also include CUGBP2 which along with Apobec-1 binds to the consensus binding sequence UUUN (A/U) U, present in c-myc, VEGF and Cyclooxygenase-2 (COX2). Myotonic dystrophy (DM), a disease associated with an unstable trinucleotide CTG repeat located in the myotonin protein kinase gene (DMPK), whereby expanded CTG (CUG) repeats result in gain of function of specific RNA-binding proteins that could regulate RNA processing in multiple tissues. A novel RNA-binding protein, CUGBP1, which specifically binds to CUG repeat sequences. CUGBP has been suggested as a candidate for an RNA-binding protein that regulates a number of RNAs by binding to CUG repeats in regulatory regions of different mRNAs. (CUGBP) exhibited no binding to an RNA oligonucleotide of triplet repeats of the same length but having a different sequence, CGG. The CUG-binding protein was localized to the cytoplasm, whereas double-stranded DNA binding proteins were localized to the nuclear extract. Rat CUG-BP1 (513aa, mouse/human 486aa, chromosome 11p11) also known as CUG-BP1, RNA-binding protein BRUNOL-2, Deadenylation factor CUG-BP, 50kD Nuclear polyadenylated RNA-binding protein, EDEN-BP, is expressed in most tissues. Applications: ELISA: 0.5-1ug/ml; Control peptide can be used to coat ELISA plates at 1ug/ml. Western Blot: 1:1000-1:5000 using ECL technique. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice