C7947-28
CTBS (Chitobiase, Di-N-acetylchitobiase)
Size
£837.00
- SKU:
- C7947-28
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- NS0-derived recominant protein corresponding to Thr39-Arg385 from human CTBS.
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- CTBS (Di-N-acetylchitobiase; also chitobiase) is a 40kD lysosomal glycoprotein that belongs to glycosyl hydrolase family # 18. It is a reducing-end glycoside hydrolase that serves to degrade asparagines-linked carbohydrates. Mature human CTBS is 347 amino acids (aa) in length and contains one catalytic domain (aa 115-357). There are multiple potential isoforms of CTBS. One shows a three aa deletion of aa 30-32, accompanied by a two two aa substitution for the C-terminal 279 amino acids. There are two potential alternate start sites, one at Met92 and a second at Met207. Mature human CTBS (aa 39-385) is 85% aa identical to mouse CTBS. Applications: Suitable for use in Direct ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.1ug/ml with recombinant human Chitobiase/CTBS. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice