C7945-02E

Csk (C-Terminal Src Kinase, C-Src Kinase, c-Src Tyrosine Kinase, Tyrosine Protein Kinase CSK, Protein-Tyrosine Kinase CYL)

Size

£614.00

SKU:: C7945-02E
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Bacterially expressed recombinant protein fragment corresponding to aa330-450 of human Csk.
Clone:: CSK-04
Format:: Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Extra Details:: C-terminal Src kinase (Csk) is a non-receptor protein tyrosine kinase. One of its major functions is to specifically phosphorylate a conserved C-terminal tyrosine of Src family kinases thereby down-regulating their kinase activity. CSK is expressed ubiquitously as a protein of approximately 50kD in cytosol, but a fraction distributes to lipid rafts due to interaction with the lipid raft-associated protein PAG (Csk-binding protein). Applications: Suitable for use in Western Blot, Immunocytochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:250-1:300. Weak non-specific band of ~60kD is detected in human cell lines at lower antibody dilutions. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.