Crystallin AB (Alpha crystallin B chain, CryAB, Alpha (B)-crystallin, Rosenthal fiber componet, Heat-shock protein beta-5, HspB5, CryA2, AACRYA, Alpha crystallin B chain, CRYA2, Crystallin alpha polypeptide 2, CTPP 2, CTPP2, Heat shock 20kD like protein, Heat shock protein beta 5, HSPB5, Rosenthal fiber component)

Size

£518.00

SKU:: C7944-60
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to the sequence near the C-terminus of human αA- and αB-Crystallin.
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
Extra Details:: Alpha-crystallin is one of the major structural proteins in the vertebrate eye lenses (2). It occurs as large aggregrates of up to 106D composed of two gene products, alphaA-and alphaB-crystallin. These two subunits have a sequence homology of approximately 57%. In humans, the alphaA-crystallin gene encodes a polypeptide of 173 residues and the alphaB-crystallin gene encodes a 175 residue polypeptide. They are related to the small heat proteins (sHspís) (3). The alphaB-crystallin protein, like sHspís, is induced by heat and osmotic shock (4). In addition, alpha-crystallin and sHspís protect other proteins against heat-induced aggregation (6). The two other crystallin families, ß and gama, are homologous to each other but not to the alpha family or the sHspís. Applications: Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot (Colorimetric): 1:1,000 Optimal dilutions to be determined by researcher. Positive Controls: Bovine alpha-Crystallin Protein Bovine alphaA-Crystallin Protein Bovine alphaB-Crystallin Protein Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice