Cryptosporidium parvum (FITC)

Size

£512.00

SKU:: C7944-16A
Purity:: Purified IgG fraction to a purity of > 95%.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Oocysts purified from bovine feces.
Format:: Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
Extra Details:: Members of the genus Cryptosporidium are parasites of the intestinal tracts of fishes, reptiles, birds and mammals. It seems that members of this genus do not display a high degree of host specificity, so the number of species in this genus remains a matter of some discussion. Cryptosporidium isolated from humans is now referred to as C. parvum. Cryptosporidium infections have been reported from a variety of wild and domesticated animals. Cryptosporidiosis is now recognized as an important opportunisitic infection, especially in immunocompromised hosts. Cryptosporidium is a small protozoan parasite, measuring about 3-5 µm. It lives on (or just under) the surface of the cells lining the small intestine, reproduces asexually, and oocysts are passed in the feces. Transmission of the infection occurs via the oocysts. Many human infections have been traced to the contamination of drinking water with oocysts from agricultural "run-off" (i.e., drainage from pastures), so it is considered a zoonosis. The most common symptom of cryptosporidiosis is watery diarrhea; other symptoms include stomach cramps or pain, fever, nausea and vomiting. In persons with healthy immune systems, symptoms usually last about 1 to 2 weeks. Applications: Suitable for use in FLISA and Immunofluorescence. Other applications not tested. Recommended Dilution: Immunofluorescence: 1:10-1:50. Acetone fixation of the antigen source is recommended prior to staining. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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