C7942-40
Cryptochrome 1 (CRY1)
Size
£869.00
- SKU:
- C7942-40
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Recombinant protein corresponding to Lys115-His224 from human CRY1, expressed in E. coli.
- Format:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- CRY1 is a critical component of the circadian oscillator that represses CLOCK:BMAL1 mediated transcription when translocated into the nucleus. It is a member of the DNA photolyase family but has no photolyase activity. Over the region used as immunogen, human and mouse CRY1 share 94% amino acid sequence homology. Applications: Suitable for use in Immunocytochemistry, Western Blot, Immunohistochemistry and Direct ELISA. Other applications have not been tested. Recommended Dilutions: Western Blot: 0.1ug/ml using Recombinant Human CRY1/ Recombinant Mouse CRY1 Immunohistochemistry (Frozen): 5-15ug/ml in Perfusion fixed frozen sections of mouse testis Immunocytochemistry: 5-15ug/ml in immersion fixed MOLT-4 human acute lymphoblastic leukemia cell line Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C after receipt. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice