C7925-03B
CrkL (Crk-like protein, v-crk sarcoma virus CT10 oncogene homolog (avian)-like)
Size
£863.00
- SKU:
- C7925-03B
- Application:
- WB
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Purified, E. coli-derived full length recombinant human CrkL (rhCrkL; aa 1-303; Accession# P46109).
- Clone:
- 9H105
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- CrkL is a widely-expressed 39kD adaptor protein that belongs to the Crk protein family and is abundantly expressed in cells of hematopoietic origin. Through its SH2 and SH3 domains, CrkL initiates interactions with numerous downstream proteins such as CBL, p130Cas, Paxillin, C3G, SOS, PI3K, c-Abl and BCR/Abl. CrkL is involved in such processes as adhesion, migration and the immune response. Furthermore, this adaptor protein plays an essential role in Chronic Myelogenous Leukemia (CML) and is constitutively phosphorylated at Y207 in CML neutrophils by the BCR/Abl kinase. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml. Lysates from human K562, A431 and Jurkat cells were resolved by SDS-PAGE. Following electrophoresis, proteins were transferred to an Immobilon-P membrane and immunoblotted with 1ug/ml mouse anti-CrkL Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile 40-50% glycerol, aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice