C7922-10N
Cripto (Teratocarcinoma-derived Growth Factor, Cripto Growth Factor, Epidermal Growth Factor-like Cripto Protein, Tdgf1) (APC)
Size
£774.00
- SKU:
- C7922-10N
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Recombinant protein corresponding to Arg26-Gln150 from mouse CRIPTO expressed in NS0 cells.
- Clone:
- 12K72
- Format:
- Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
- Extra Details:
- Cripto is the founding member of the epidermal growth factor-CriptoFRL1Cryptic (EGF-CFC) family of signaling proteins that function in cancer and various developmental processes. These developmental processes include: formation of the germ layers and dorsal organizer, specification of anterior-posterior and left-right axes, and differentiation of heart muscle. Cripto exists both as a secreted and as a GPI-anchored membrane protein and both the secreted and membrane bound forms of Cripto demonstrate biological activity. Cripto, also known as CFC-2 or TDGF-1 (teratocarcinoma-derived growth factor), was originally isolated from an undifferentiated human teratocarcinoma cell line as a potential oncogene. It is overexpressed in many types of cancers and acts as a growth factor for tumors. Mouse Cripto shares 66% and 34% aa identity with human Cripto and zebrafish OEP, respectively. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul to label 10e6 of D3 mouse embryonic stem cells Optimal dilutions to be determined by the researcher. Absorption: 620-650nm Emission: 660-670nm Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Shipping Conditions:
- Blue Ice