C7920-01
Cre Recombinase
Size
£403.00
- SKU:
- C7920-01
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Purified recombinant Cre recombinase protein
- Clone:
- 9E2
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 1% BSA, 0.02% sodium azide. Reconstitute with 80ul sterile ddH2O.
- Extra Details:
- Cre-recombinase is a 38kD DNA recombinase derived from the P1 bacteriophage. It is highly specific to a 34bp DNA sequence (loxP) found in P1 DNA. It catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. It is homotetramer when bound to DNA and belongs to the phage integrase family. C7920-01 is an important tool for the identification of Cre recombinase in different protein expression systems. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 0.05-0.2ug/ml Western Blot: 0.1-1ug/ml Optimal dilutions to be determined by the researcher. Hybridoma: Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice