C7915-16C

CREB1 (Cyclic AMP-responsive Element-binding Protein 1, CREB-1, cAMP-responsive Element-binding Protein 1)

Size

£685.00

SKU:: C7915-16C
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Partial recombinant protein corresponding to aa1-96 from human CREB1 (NP_004370) with GST tag. MW of the GST tag alone is 26kD.
Clone:: 5F2
Format:: Supplied as a liquid in PBS, pH 7.2.
Extra Details:: CREB is a transcription factor which binds to cyclic AMP response elements (CRE's), and is induced by phosphoyrlation on the part of PKA, CaM K IV, and other protein kinases. Activated CREB will dimerize, bind to DNA, and recruit histone acetyl transferases. Applications: Suitable for use in ELISA, Immunohistochemistry, and Western Blot. Other applications not tested. Recommended Dilution: Western Blot (transfected lysate): 1-5ug/ml detects a band at ~37kD using CREB1 transfected 293T lysate Immunohistochemistry (paraffin): 1ug/ml using human kidney tissue ELISA: 1-5ug/ml using recombinant protein. The detection limit for recombinant GST tagged CREB1 is ~1ng/ml Optimal dilutions to be determined by the researcher. AA Sequence: MTMESGAENQQSGDAAVTEAENQQMTVQAQPQIATLAQVSMPAAHATSSAPTVTLVQLPNGQTVQVHGVIQAAQPSVIQSPQVQTVQISTIAESE Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.