CREB, phosphorylated (Ser129, Ser133) (cAMP Response Element Binding Protein)

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£953.00

SKU:: C7915-08A
Purity:: Purified from rabbit serum by sequential epitope-specific chromatography. Negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated CREB. The final product is generated by affinity chromatography using a CREB-derived peptide that is phosphorylated at serines 129 and 133.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic phosphopeptide derived from a region of human CREB that contains serines 129 and 133. The sequence is conserved in mouse and rat.
Format:: Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.09% sodium azide, 50% glycerol.
Extra Details:: cAMP responsive element binding protein (CREB) is a 43kD leucine zipper transcription factor that stimulates transcription and gene expression after its phosphorylation on serine residues. CREB plays an important role in development, glucose homeostasis and growth-factor-dependent cell survival and is implicated in learning and memory. The phosphorylation of CREB at GSK-3β and PKA sites (serines 129 and 133, respectively) is essential for its transcriptional activity. Applications: Suitable for use in ELISA, Western Blotting. Other applications not tested. Recommended Dilution: Western Blotting: 1:1000-1:2000 Optimal dilutions to be determined by the researcher. Positive Control Used: NIH3T3 cells +/- PDGF, Y-1 cells. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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