C7915-07
CREB, phosphorylated (Ser133) (cAMP Response Element Binding Protein)
Size
£978.00
- SKU:
- C7915-07
- Purity:
- Supernatant purified by subsequent thiophilic adsorption and size exclusion chromatography
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- KLH-conjugated, synthetic phosphopeptide (REILSRRP(pS)YR) corresponding to amino acids 125-135 of human CREB. The immunizing sequence is identical in human and mouse
- Clone:
- 10E9
- Format:
- Supplied as lyophilized powder from serum-free cell culture supernatant in 30ul of 2X PBS containing PEG 3000, sucrose, 0.09% sodium azide. Reconstitute with 500ul of sterile, ddH2O to make a 0.2mg/ml solution.
- Extra Details:
- Transcription factor CREB binds the cyclic AMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth or neurotrophic factors. Protein kinase A and the calmodulin-dependent protein kinase CaMKII stimulate CREB phosphorylation at Ser133, a key regulatory site controlling transcriptional activity (1,2). Phosphorylation at Ser133 is also mediated by p44/42 MAP kinase, p90RSK, p38 MAP kinase and MSK1 (3-5). CREB appears to play an important role in learning and memory in both flies and mice. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5-2ug/ml detected phosphorylated CREB in whole cell lysates from HepG2 and PC12 cells treated with forskolin. Optimal dilutions to be determined by the researcher. Control: Forskolin-treated PC12 cell lysate *Recommended secondary antibodies: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-14C: IgG, Fc (HRP) Pab Gt xMo I1904-19C: IgG, F(ab’)2 (HRP) Pab Gt xMo Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice