C7915-02B
CREB (cAMP Response Element Binding Protein)
Size
£774.00
- SKU:
- C7915-02B
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Phosphorylated peptide fragment of CREB (pS133), conjugated with KLH.
- Clone:
- p-S133
- Format:
- Supplied as a lyophilized powder in 0.01M PBS, pH 7.2. Reconstitute with 500ul sterile ddH2O.
- Extra Details:
- The transcription factor cAMP response element binding (CREB) binds the palindromic octanucleotide TGACGTCA. Members of ATF/CREB family include CREB-1, CREB-2 (also designated ATF-4), ATF-1, ATF-2 and ATF-3. These proteins share highly conserved leucine zipper dimerization and basic DNA binding domains at the COOH terminal but are highly divergent in their amino terminal domains. CREB-mediated transcription regulates diverse cellular responses, including intermediary metabolism, neuronal signaling, cell proliferation, and apoptosis. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Indirect ELISA: coating quantity is 1ug/ml of peptide/or peptide conjugate in pH 9.5 carbonate buffer, 100ul/well. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice