C7910-27V

Creatine Kinase MM Isoenzyme I, Recombinant (CKMM) (Pichia)

Size

£887.00

SKU:: C7910-27V
Purity:: ~95% (SDS-PAGE). Purified under non-denaturing conditions.
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 0.01M Tris-HCl, pH 7.0-7.5, 0.075M sodium chloride, 10mM beta-mercaptoethanol, 0.09% sodium azide, 50% glycerol.
Extra Details:: Recombinant full length Creatine Kinase MM isoenzyme without the C-terminal lysine on both subunits. CK-MM is a 47kD dimeric protein comprised of 2 identical subunits of Type 1 sequence. Purified in the enzymatically active form. Reacts with polyclonal antibodies specific to MM isoenzyme in ELISA. Applications: Suitable for use in ELISA and Western Blot. Major band migrating above 37kD in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Unit Definition: One unit will transfer one micromole of phosphate from creatine phosphate to ADP per minute at 37°C. Measured at 340nm as one equimolar amount of NADH produced by coupled reaction. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.