C7910-16H

CK-BB, NT (CKBB, Creatine Kinase B-type, B-CK, Creatine Kinase B Chain, CKB)

Size

£819.00

SKU:: C7910-16H
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Synthetic 17-mer peptide corresponding to aa4-20, SNSHNALKLRFPAEDEF, from human creatine kinase brain-type, at N-terminal.
Clone:: 13A41 (CK-BYK/21E10)
Format:: Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
Extra Details:: Human CKB is a protein of 381aa (~45kD), expressed in a number of tissues. CKB is most abundant in adult brain, approx. 5-fold lower in the stomach, 10-fold lower in the heart and barely detectable in liver. In brain, whereas most CKB has been shown to be cytosolic, several of the reactions requiring CKB are membrane-associated. CKB belongs to the creatine kinase (CK) isoenzymes that catalyse the synthesis of phosphocreatine (PCr) and its subsequent use in the regeneration of ATP in cell types where the consumption of ATP is rapid and/or sudden. Expression of CKB is developmentally controlled: in rat, brain CKB protein at birth is extremely low and increases 10-fold until week 4. This reflects the many energy-demanding processes in brain during brain development. Applications: Suitable for use in Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:50 (reduced; size ~43-46kD) Immunohistochemistry (Frozen): fixed 2hr in 2% paraformaldehyde and 0.1 % glutaraldehyde in 0.1M PB; stored until use in 1% paraformaldehyde; blocking with 1% normal swine serum Immunohistochemistry (Paraffin): antigen retrieval: 0.5% pepsin in 0.01N HCl for 15min at 37°C; endogenous peroxidise blocking by using 3% H2O2 in PBS for 30min; blocking with 1% normal swine serum for 30min. Optimal dilutions to be determined by the researcher. Positive Control: Brain lysate, Human Purkinje cells Negative Control: Human fibroblasts. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice