C7910-10J

Creatine Kinase BB Isoenzyme, Recombinant (CKBB)

Size

£918.00

SKU:: C7910-10J
Purity:: ~95% (SDS-PAGE). Purified under non-denaturing conditions.
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 10mM Bis-Tris-HCl, pH 6.0-0.2, 0.5mM DTT, 0.5mM EDTA, 50% glycerol. No preservative added.
Extra Details:: Full length creatine kinase BB isoenzyme with aa sequence identical to the native enzyme. CK-BB is a 47kD dimeric protein comprised of 2 identical B subunits. Purified in the enzymatically active form. Reacts with polyclonal antibodies to BB isoenzyme in ELISA. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Unit Definition: One unit will transfer one micromole of phosphate from creatine phosphate to ADP/minute at 37°C. Measured at 340nm as one equimolar amount of NADH produced by coupled reaction. Storage and Stability: Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.